What is RNA interference?RNA interference (RNAi) is the process of silencing a gene through the use of double stranded RNA. Double stranded RNA serves as a guide leading RNAi machinery to destroy the messenger RNA that codes for a specific gene. RNAi is used in research due to it's powerful ability to knock down the activity of a targeted gene very quickly. The use of RNAi helps to reveal the genes involved in normal and disease processes [1].
Many databases are available to identify the mutant the phenotypes caused by the knock out of the homologous PRKAG2 gene. These databases are organism-specific and include: Mouse Genome Informatics (mouse), FlyBase (fruit fly), ZFIN (zebrafish), and WormBase (C. elegans). |
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What are the PRKAG2 model organism RNAi mutant phenotypes?
Mus musculus (Mouse)
MGI did not produce any insightful results on abnormal phenotypes reported in the mutated prkag2 homologous gene. There may have been an insignificant number of RNAi experiments done on the mouse prkag2 gene or the experiments did not produce varied phenotypes.
MGI did not produce any insightful results on abnormal phenotypes reported in the mutated prkag2 homologous gene. There may have been an insignificant number of RNAi experiments done on the mouse prkag2 gene or the experiments did not produce varied phenotypes.
Drosophila melanogaster (Fruit Fly)
The listed phenotypes were found using FlyBase. |
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Danio rerio (Zebrafish)
ZFIN did not produce any insightful results on abnormal phenotypes reported in the mutated prkag2 homologous gene. There may have been an insignificant number of RNAi experiments done on the homologous zebrafish prkag2 gene or the experiments did not produce varied phenotypes.
ZFIN did not produce any insightful results on abnormal phenotypes reported in the mutated prkag2 homologous gene. There may have been an insignificant number of RNAi experiments done on the homologous zebrafish prkag2 gene or the experiments did not produce varied phenotypes.
Caenorhabditis elegans (Worm)
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Analysis
It is very important to conduct research on model organisms because they give us unique insights into the diseased phenotype in humans. The mutant phenotypes seen in fruit flies and worms may help us to understand the dynamic function of the prkag2 gene. It would be helpful to have more extensive research conducted in mice and zebrafish because it may also produce some insightful results. Based on the high similarity between human and mice cardiac anatomy it would be very crucial to conduct research on this model organism.
References
[1] "RNA Interference Fact Sheet." National Institute of General Medical Sciences. November 2012. Web. May 10 2013. <http://www.nigms.nih.gov/News/Extras/RNAi/factsheet.htm>
[2] Eppig JT, Blake JA, Bult CJ, Kadin JA, Richardson JE; the Mouse Genome Database Group. 2012. The Mouse Genome Database (MGD): comprehensive resource for genetics and genomics of the laboratory mouse. Nucleic Acids Res 2012; 40(1):D881-86.
[3] S.J. Marygold, P.C. Leyland, R.L. Seal, J.L. Goodman, J.R. Thurmond, V.B. Strelets, R.J. Wilson and the FlyBase Consortium (2013).FlyBase: improvements to the bibliography. Nucleic Acids Res. 41(D1):D751-D757.
[4] Sprague, J., L. Bayraktaroglu, D. Clements, T. Conlin, D. Fashena, K. Frazer, M. Haendel, D. Howe, P. Mani, S. Ramachandran, K. Schaper, E. Segerdell, P. Song, B. Sprunger, S. Taylor, C. Van Slyke and M. Westerfield. (2006) The Zebrafish Information Network: the zebrafish model organism database. Nucl. Acids Res. 34, D581-D585.
[5] http://www.wormbase.org/db/get?name=WBGene00013732;class=Gene
[2] Eppig JT, Blake JA, Bult CJ, Kadin JA, Richardson JE; the Mouse Genome Database Group. 2012. The Mouse Genome Database (MGD): comprehensive resource for genetics and genomics of the laboratory mouse. Nucleic Acids Res 2012; 40(1):D881-86.
[3] S.J. Marygold, P.C. Leyland, R.L. Seal, J.L. Goodman, J.R. Thurmond, V.B. Strelets, R.J. Wilson and the FlyBase Consortium (2013).FlyBase: improvements to the bibliography. Nucleic Acids Res. 41(D1):D751-D757.
[4] Sprague, J., L. Bayraktaroglu, D. Clements, T. Conlin, D. Fashena, K. Frazer, M. Haendel, D. Howe, P. Mani, S. Ramachandran, K. Schaper, E. Segerdell, P. Song, B. Sprunger, S. Taylor, C. Van Slyke and M. Westerfield. (2006) The Zebrafish Information Network: the zebrafish model organism database. Nucl. Acids Res. 34, D581-D585.
[5] http://www.wormbase.org/db/get?name=WBGene00013732;class=Gene
Margaret Beatka ([email protected])
Page Last Updated: 5/10/13
This web page was produced as an assignment for Genetics 677, as an undergraduate course at UW-Madison.
Page Last Updated: 5/10/13
This web page was produced as an assignment for Genetics 677, as an undergraduate course at UW-Madison.