What are micoarrays?
DNA microarray technology is a molecular tool that involves providing a specific DNA template and observing which mRNA molecule binds to it, or hybridizes it. This process allows scientists to test the expression level of thousands of genes in a cell very rapidly all at once. Micoarrays can give insights into changes in gene expression levels, genomic gains and losses, and mutations in DNA. This technology allows researchers to hypothesize the function of new genes based on expression patterns of already known genes. The use of DNA microarray technology has also sped up the identification of specific disease associated genes due to it's ability to examine multiple genes at once [1].
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What microarray experiments have been performed for the prkag2 gene?
The GEO database was used to view what kind of microarrays have been performed on the human prkag2 gene. It appears that microarrays have been performed to study human prkag2 in regards to coronary collateral artery growth, sickle cell anemia, alcoholic hepatitis, inflammatory dilated cardiomyopathy, muscle function and aging, and much more. The expression of prkag2 in different diseases and tissues seems to be well studied in rat and mouse model organisms. The data on the right shows the expression measurement of the homologous prkag2 gene in the heart atrium and ventricle tissue of four different rats. This data suggests that prkag2 has a higher expression in the heart atrium than it does in heart ventricles. The continued use of microarray experiments in humans and other model organisms will give more insight into the localization of the prkag2 gene and any other diseases that it might be associated with.
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References
[1] "Microarrays: Chipping Away at the Mysteries of Science and Medicine." A Science Primer. National Center for Biotechnology Information. 27 July 2007. Web. 12 May 2013. <http://www.ncbi.nlm.nih.gov/About/primer/microarrays.html>
[2] Barrett, T., Wilhite, S.E, Ledoux, P., et al. (2013). NCBI GEO: archive for functional genomics data sets--update. Nucleic Acid Res. 2013 Jan; 41(Database issue):D991-5.
[2] Barrett, T., Wilhite, S.E, Ledoux, P., et al. (2013). NCBI GEO: archive for functional genomics data sets--update. Nucleic Acid Res. 2013 Jan; 41(Database issue):D991-5.
Margaret Beatka ([email protected])
Page Last Updated: 5/13/13
This web page was produced as an assignment for Genetics 677, as an undergraduate course at UW-Madison.
Page Last Updated: 5/13/13
This web page was produced as an assignment for Genetics 677, as an undergraduate course at UW-Madison.